首页> 外文期刊>Advances in Microbiology >Adhesion of Gallibacterium anatis to Chicken Oropharyngeal Epithelial Cells and the Identification of Putative Fimbriae
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Adhesion of Gallibacterium anatis to Chicken Oropharyngeal Epithelial Cells and the Identification of Putative Fimbriae

机译:鸡鸡口咽上皮细胞粘附干an虫和假定菌毛的鉴定

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Microbial infections are typically initiated by the colonization of tissues by a specific mechanism that promotes adherence to host cells or tissues. In this work, we characterized the ability of Gallibacterium anatis F149T to express fimbriae that may be involved in mucosal attachment. Using transmission electron microscopy, the fimbriae-like structures could be observed on the surface of negatively stained G. anatis F149T, and these structures were further visualized after being released by physical shaking. When the fimbriae-like structures were separated by SDS-PAGE, the proteins comprising them were isolated and sized at 13 and 25 kDa. G. anatis F149T was able to adhere to chicken oropharyngeal epithelial cells. Adhesion could be completely inhibited by pretreatment of the bacterial cells with trypsin, whereas 25% inhibition was attained after pretreatment with an antiserum against the 13 kDa protein. We demonstrated by immuno-gold electron microscopy that the antibodies from the antiserum were specifically associated with the fimbria-like structures on G. anatis. These results indicated that G. anatis F149T expresses fimbriae that contribute to its adhesion to chicken oropharyngeal epithelial cells and may be important for colonization of the upper respiratory tract.
机译:微生物感染通常是由组织定植引起的,该定植是通过促进对宿主细胞或组织的粘附的特定机制进行的。在这项工作中,我们表征了Analitis F149T菌表达可能参与粘膜附着的菌毛的能力。使用透射电子显微镜,可以在染色阴性的G. anatis F149T的表面上观察到菌毛状结构,并通过物理摇动将其释放后进一步可视化。当通过SDS-PAGE分离菌毛样结构时,分离出包含它们的蛋白质,并以13kDa和25kDa定尺寸。 G. anatis F149T能够粘附于鸡口咽上皮细胞。通过用胰蛋白酶预处理细菌细胞可以完全抑制粘附,而在用针对13 kDa蛋白的抗血清预处理后达到25%的抑制率。我们通过免疫金电子显微镜证明,来自抗血清的抗体与G. anatis上的菌毛样结构特异性结合。这些结果表明,Anatis F149T表达菌毛,该菌毛有助于其与鸡口咽上皮细胞的粘附,并且对于上呼吸道的定殖可能是重要的。

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