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首页> 外文期刊>Advanced Pharmaceutical Bulletin >Evaluating Inhibitory Effects of Paclitaxel and Vitamin D3 Loaded Poly Lactic Glycolic Acid Co-Delivery Nanoparticles on the Breast Cancer Cell Line
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Evaluating Inhibitory Effects of Paclitaxel and Vitamin D3 Loaded Poly Lactic Glycolic Acid Co-Delivery Nanoparticles on the Breast Cancer Cell Line

机译:评价紫杉醇和维生素D3载聚乳酸乙醇酸共传递纳米粒子对乳腺癌细胞株的抑制作用。

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Purpose: Paclitaxel (PTX) has transpired as a significant agent in the treatment of breast cancer. Meanwhile, polylactic glycolic acid (PLGA) nanoparticles (NPs) are able to increase the anticancer effect of the PTX in the blood. Methods: Nano-precipitation was used to prepare the PLGA-PTX-VitD3 co-delivery NPs. Drug loading, encapsulation efficiency, in vitro release profile, cell viability, migration, apoptosis, and bcl2 expression of NPs were evaluated. Results: The average size of co-delivery NPs was 231 ± 46 nm. Observed was a controlled release of the PTX and vitamin D3 from co-delivery NPs between 0.5 and 240 hours. MTT showed the ability of 8 μg.mL-1 of co-delivery NPs to kill 50 % of the MCF-7; likewise, the co-delivery NPs prevented MCF-7 migration. The co-delivery NPs led 46.35 % MCF-7 to enter primary apoptosis. 60.8% of MCF-7 in the control group were able to enter the G (1) phase of the cell cycle. The co-delivery NPs increased expression of bax. In addition to its higher toxicity against MCF-7 than that of PTX, co-delivery NPs were able to release drugs continuously for a long period, which indeed increased the efficiency of the drugs. Conclusion: The effect of co-delivery NPs on MCF-7 cell viability was different from that in other drugs. In fact, the co-deliver NPs were able to release drugs continuously for a long time, this could induce primary apoptosis in the MCF-7 and decrease the metastasis and toxicity of drugs.
机译:目的:紫杉醇(PTX)已成为治疗乳腺癌的重要药物。同时,聚乳酸乙醇酸(PLGA)纳米颗粒(NPs)能够增强PTX在血液中的抗癌作用。方法:采用纳米沉淀法制备PLGA-PTX-VitD3共递送NPs。评估了NPs的载药量,包封效率,体外释放曲线,细胞活力,迁移,凋亡和bcl2表达。结果:共递送NP的平均大小为231±46nm。观察到在0.5到240小时之间,PTX和维生素D3从共同释放的NP中受控释放。 MTT显示8μg.mL-1的共同释放NP杀死50%的MCF-7的能力;同样,共同交付NP阻止了MCF-7迁移。共递送NP导致46.35%的MCF-7进入原代细胞凋亡。对照组中60.8%的MCF-7能够进入细胞周期的G(1)期。共递送NP增加了bax的表达。除对MCF-7的毒性比对PTX的毒性更高外,共递送NP还能够长时间连续释放药物,这确实提高了药物的效率。结论:共递送NPs对MCF-7细胞活力的影响与其他药物不同。实际上,共递送NP能够长时间连续释放药物,这可以诱导MCF-7的原代细胞凋亡并降低药物的转移和毒性。

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