In-Vitro Release Characteristics of Hydrophobic Breast Cancer Drug Loaded Poly Lactic-co-Glycolic Acid (PLGA) Nanoparticles

摘要

The present work focuses on the development of biodegradable PLGA nanoparticles (NPs) for controlled release of a breast cancer drug, letrozole. NPs of different drug-polymer ratio formulations (F1, F2, F3, F4) were fabricated using solvent evaporation technique. Physicochemical characteristics of these NPs were assessed using dynamic light scattering (DLS) spectrophotometer. In-vitro drug release study was carried out over an extended period of 30 days at 37°C in simulated physiological fluid. To evaluate the release kinetics, data was fitted to different models. NPs with various sizes and size distributions were obtained by altering the drug-polymer ratio. Zeta potential of PLGA and drug loaded NPs were found to be -29.4±1.3 mV and -21.0±0.6 mV, respectively. The release kinetics of the drug from NPs was in good agreement with Korsmeyer-Peppas model, ensuring controlled release of the drug from the NPs. In-vitro release studies showed high correlation coefficient (R~2 = 0.90) for formulation F2 and F3 up to 30 days. It is concluded that NPs with F2 and F3 formulations provide a controlled release of the incorporated drug and, therefore, hold promise to be investigated further in detail.