Characterisation of the promoter of Medicago sativa cyclin dependent kinase - Cdc2MsF

摘要

The eukaryotic cell cycle is regulated at multiple points and most of these involve the activation of a special class of serine-threonine protein kinases, which functionally require binding for activity to a regulatory protein known as a cyclin and are therefore named cyclin-dependent kinases (CDKs). Alfalfa (Medicago sativa L.) is one of the most important forage crops and it is used as a model plant in molecular biology. It was demonstrated that at least six Cdc2 homologous genes, members of the CDK family, are expressed in tetraploid alfalfa (Magyar et al., 1997). The mRNA level of one of them – cdc2MsF – showed a peak during G2/M phases. Our aim is to characterize the cdc2MsF promoter (fpr), which would develop further the study of the cell cycle.