Developing a novel method to identify genes involved in germ line induction of Drosophila melanogaster embryos

摘要

Embryonic germ cell development in Drosophila melanogaster depends on the germ plasm, the most posterior part of the ooplasm. There are experimental evidences that germ plasm contains all the factors necessary for the formation of the embryonic germ cells (Mahowald 2001). Though most of the germ plasm components have not been identified yet, it is known that at least in part the germ line factors are stored in the form of RNA (Tomancak et al. 2002). The aim of our work was to identify novel germ plasm-enriched, localized RNAs by combining cDNA microarray and RNA in situ hybridization techniques and to verify the results with a functional analysis.