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首页> 外文期刊>Cytotechnology >Evaluation of the new serum-free medium (MDSS2) for the production of different biologicals: Use of various cell lines
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Evaluation of the new serum-free medium (MDSS2) for the production of different biologicals: Use of various cell lines

机译:评估用于生产不同生物的新型无血清培养基(MDSS2):各种细胞系的使用

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摘要

The presence of serum in cell culture raises safety problems for the production of biologicals, thus a new serum-free medium (MDSS2) was developed. The evaluation of this medium for the growth of different cell lines (BHK-21 C13, BSR and Vero) has shown that cells grew in this medium similarly to standard serum-containing medium, independently of the culture system used: in static (as monolayer) as well as in agitated systems (in suspension in spinner and perfusion reactors). BHK-21 and BSR cells grew as aggregate cultures and could proliferate in both static and agitated culture systems. Vero cells stayed attached to a substrate and proliferated equally in static and in agitated microcarrier-culture systems. The cell densities obtained with BHK-21 cells depended only on the culture system used. They ranged from 2–3×106 to 6–12×106 cells per ml for static batch and perfusion reactor cultures respectively. The cell concentration was 3 to 6 times higher than in classical cultures performed in serum-containing medium. The cell densities obtained with Vero cells were indistinguishable from those obtained in serum-containing medium, whatever the cell culture system used. These cell lines have been used for the production of rabies virus. With respect to BHK-21 and BSR, similar production rates of rabies glycoprotein have been found as in the standard roller bottle process. The production of rabies virus and of viral glycoprotein by Vero cells cultivated in serum-free medium was augmented 1.5-fold and 2.5-fold, respectively, when compared to serum-containing medium.A recombinant BHK-21 cell line, producing human IL-2, can also proliferate in MDSS2, after addition of insulin. The specific IL-2 production rate was augmented 3–4 fold in comparison to serum-containing medium.For the cells tested, the MDSS2 serum-free medium is a good growth and production medium. Its use for cultivating other cell lines and/or for the production of other biologicals is discussed.
机译:细胞培养物中血清的存在增加了生物制剂生产的安全性问题,因此开发了一种新的无血清培养基(MDSS2)。对这种培养基用于不同细胞系(BHK-21 C13,BSR和Vero)生长的评估表明,该培养基中的细胞生长类似于含标准血清的培养基,独立于所用的培养系统:静态(单层) )以及搅拌系统(在旋转器和灌注反应器中悬浮)。 BHK-21和BSR细胞作为聚集培养物生长,并且可以在静态和搅拌培养系统中增殖。 Vero细胞保持附着在基质上,并在静态和搅拌微载体培养系统中均等增殖。用BHK-21细胞获得的细胞密度仅取决于所用的培养系统。对于静态分批培养和灌注反应器培养,它们的范围为每毫升2–3×106至6–12×106细胞。细胞浓度比在含血清的培养基中进行的经典培养高3至6倍。无论使用何种细胞培养系统,用Vero细胞获得的细胞密度都与在含血清的培养基中获得的细胞密度没有区别。这些细胞系已用于生产狂犬病毒。关于BHK-21和BSR,已经发现狂犬糖蛋白的生产速率与标准滚瓶工艺相似。与不含血清的培养基相比,在无血清培养基中培养的Vero细胞产生的狂犬病病毒和病毒糖蛋白的产量分别比含血清的培养基增加1.5倍和2.5倍。重组BHK-21细胞系产生人IL- 2,添加胰岛素后,还可在MDSS2中增殖。与含血清的培养基相比,特定的IL-2生产率提高了3-4倍。对于所测试的细胞,MDSS2无血清培养基是良好的生长和繁殖培养基。讨论了其在培养其他细胞系和/或生产其他生物中的用途。

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