BHK 21?C13?cells for Aujeszky’s disease virus production using the multiple harvest process

摘要

Production of Aujeszky’s disease virus (ADV) from BHK 21?C13 suspension cells using a simple harvest and multiple harvest process mode was examined. We studied growth kinetics of BHK 21?C31?cells in 750?ml spinner flask containing 500?ml of culture medium. In the simple harvest process of ADV production, 425?ml of virus harvest was obtained with a virus titer of 106.4 TCID50?ml?1 which corresponds to 10,676 doses of vaccine. The multiple harvest process resulted in 850?ml of virus harvest with a virus titer of 106.5 TCID50?ml?1 corresponding to 26,877 AD vaccine doses. In conclusion, the multiple harvest process mode using BHK 21?C13 can be considered as a favorable process to produce ADV.