Screening of microorganisms for production of carotenoids Selecci???3n de microorganismos para la producci???3n de carotenoides

摘要

This work aimed at screening microorganisms capable of producing carotenoids. Microorganisms were isolated from soil, leaves, fruits, flowers, agro-industrial wastes, and processed product in decomposition and then grown in an orbital shaker, using 10% (w/v) of inoculum (25???????°C, 180????rpm for 48????h) and incubated for 120????h in a dark room. A total of 116 microorganisms were isolated and screened: 16 yeasts, 65 bacteria, and 35 fungi. Three yeasts demonstrated great potential to produce red carotenoids, with a total carotenoid content of 707?￠????818????????g L ?￠????1 (99?￠????263????????g g ?￠????1 ). One filamentous fungus and two bacteria produced mostly yellow pigments in a final concentration ranging from 1063 to 2563????????g L ?￠????1 (239?￠????2310????????g g ?￠????1 ). From 116 isolated microorganisms, several of them showed potential for production of carotenoid-synthesizing activity. RAPD analysis was used to check the genetic differences between the screened strains, showing that all microorganisms were from different species. El objetivo de este trabajo fue examinar los microorganismos capaces de producir carotenoides. Microorganismos de tierra, hojas, frutos, flores, residuos agro-industriales y producto procesado en descomposici???3n se aislaron y se cultivaron en un agitador orbital, usando 10% (w/v) de in???3culo (25???????°C, 180 rpm durante 48 horas) e incubados durante 120 h en una habitaci???3n oscura. Se aislaron y examinaron un total de 116 microorganismos, 16 levaduras, 65 bacterias y 35 hongos. Tres levaduras demostraron un gran potencial para producir carotenoides rojos, con un contenido total de carotenoides de entre 707????a 818 ????g L ?￠????1 (de 99????a 263 ????g g ?￠????1 ). Un hongo filamentoso y dos bacterias produjeron en su mayor???-a pigmentos amarillos en una concentraci???3n final de entre 1063 a 2563????????g L ?￠????1 (239????a 2310????????g g ?￠????1 ). De 116 microorganismos aislados, varios de ellos mostraron potencial para la producci???3n de actividad sintetizadora de carotenoides. Se us???3 el an????lisis RAPD para revisar las diferencias gen????ticas entre las cepas examinadas, mostrando que todos los microorganismos eran de diferentes especies.