Artificial propagation of African catfish ( Clarias gariepinus ): the application of a single dose of pellets containing D-Ala6,Pro9NEt-mGnRH and dopamine inhibitor metoclopramide

摘要

The effects on reproduction of African catfish ( Clarias gariepinus Burchell 1822) were investigated in three experiments conducted under controlled conditions, carp pituitary (at the dose of 4 mg/kg body weight) or Ovopel – a preparation that contains a mammalian GnRH analogue D-Ala6,Pro9NEt-mGnRH (1 pellet/kg body weight) and dopamine receptor antagonist metoclopramide (10 mg/kg) being used as ovulation stimulators. The application of Ovopel induced the statistically significantly ( P ≤ 0.01) higher weight of eggs per kg female body weight and the statistically significantly ( P ≤ 0.05) higher quality of eggs after 24 h incubation in comparison with the effects of hypophysation. No effect of the experiment on the weight or quality of obtained eggs was determined while the interaction between the experiment and the ovulation stimulator was statistically significant ( P ≤ 0.05) with respect to the percentage of egg fertilization. Statistically significant ( P ≤ 0.05) correlation was found between the percentage of egg fertilization and that of living embryos, the determined correlation coefficient being higher after the application of Ovopel than after the carp pituitary homogenate.