Cryoprotection synergism between glycerol and dimethyl sulfoxide improves the mitochondrial transmembrane potential, plasmalemma, acrosomal and DNA integrities, and in vivo fertility of water buffalo (Bubalus bubalis) spermatozoa

摘要

The objective of the study was to devise a cryoprotection synergism between glycerol and dimethyl sulfoxide (DMSO) for water buffalo spermatozoa. Additionally, the effect of best evolved concentrations of glycerol and DMSO in extender was assessed on in vivo fertility of buffalo spermatozoa. Ejaculates (n?=?30) were equally distributed into five aliquots; first aliquot was diluted at 37?°C in extender having 7?% glycerol (control); second aliquot was diluted at 37 °C as well as at 4?°C in extender having 3.5?% DMSO (Group 1); third aliquot was diluted at 37?°C in extender having 3.5?% glycerol and then at 4?°C in extender having 3.5?% DMSO (Group 2); fourth aliquot was diluted at 37?°C in extender having 3.5?% DMSO and then at 4?°C in extender having 3.5?% glycerol (Group 3); fifth aliquot was diluted in extenders having 1.75?% glycerol and 1.75?% DMSO at 37 as well as at 4?°C (Group 4). At post thawing, sperm progressive motility (%), rapid velocity (%), average path velocity (μm/s), curved line velocity (μm/s), in vitro longevity (%), structural and functional integrity of plasmalemma (%), mitochondrial transmembrane potential (%) and viable sperm with intact acrosome (%) were higher (P?