Comparison of antioxidative activity data for aqueous extracts of lemon balm (Melissa officinalis L.), oregano (Origanum vulgare L.), thyme (Thymus vulgaris L.), and agrimony (Agrimonia eupatoria L.) obtained by conventional methods and the DNA-based biosensor

摘要

The antioxidative pr oper ties of aqueous plant extracts were evaluated using common methods such as the Rancimat and 2,2'-diphe ny l-1-picrylhydrazy l (DPPH) free r adical me thod. More over, a voltamme tric procedure based on the pro tective effect of antioxidants against the oxidative DNA damage was employe d using a disposable DNA b iose nsor fabricated as a scree n-printed e lectrode chemically modified by calf thy mus double stranded (ds) DNA. The total polyphenols wer e also de ter mined spe ctrophotometr ically with the Folin-Ciocalteu age nt. The e xtracts o f ore gano and le mon balm exhib ited significantly higher activity than tho se o f thyme and agrimo ny. The r esults were treated statistically and their oper ational characte r is discussed.