Baicalin Regulates Neuronal Fate Decision in Neural Stem/Progenitor Cells and Stimulates Hippocampal Neurogenesis in Adult Rats

摘要

Summary Background Recent studies revealed that baicalin, a flavonoid compound derived from the root of S cutellaria baicalensis G eorgi , could promote neuron differentiation of NSPC s after commencing the differentiation process in vitro . However, this may not be the most efficacious strategy to determinate cell fate. Here, we have investigated whether baicalin can influence early events of neuron generation and stimulate adult neurogenesis. Results Transient exposure of NSPC s to baicalin during proliferation could activate M ash1 to alter the differential fate and increase the proportion of cells expressing neuronal markers. Seven days after, rats were exposed to transient cerebral ischemia, they were treated for 3 weeks with baicalin, B rd U labeling study showed that exposure to baicalin increased the number of newly generated cells in hippocampus, B rd U / N eu N double staining analysis indicated that baicalin could promote new neuron production after cerebral ischemia. Additionally, M orris water maze test showed that delayed postischemic treatment with baicalin improved cognitive impairment. Conclusions These results identify the existence of a single molecule, baicalin, which can specify the neuronal fate of multipotent NSPC s and stimulate neurogenesis, making it a promising candidate for developing clinically relevant strategies to manipulate neuronal fate of NSPC s for brain repair.