Choline administration attenuates aspects of the dystrophic pathology in mdx mice

摘要

Background & aimsDystrophic muscle fibres are fragile and prone to breakage, leading to impaired Ca2+homeostasis and excessive inflammation, resulting in muscle wasting and weakness. Choline, an essential water-soluble nutrient, is involved in multiple biological processes, including modulation of inflammation and oxidative stress. We tested the hypothesis that choline supplementation would ameliorate the dystrophic pathology inmdxmice.MethodsThree-week old malemdxmice (n?=?40) were fed control purified laboratory chow (CON; n?=?20) or a choline-enriched diet (5?g/kg choline; CHL, n?=?20) for four weeks. Rotarod performance, grip strength and running (wheel) distance were assessed during treatment. Markers of Ca2+-handling, inflammation, oxidative stress and fibrosis were measured in the diaphragm, quadriceps muscle and the liver.ResultsCholine-treatedmdxmice displayed less macrophage (CD68 -33%, P?