Effects of differences in the timing of ferrous sulfate supplementation on lipid peroxidation and activation of NF-κB in the placenta of pregnant rats

摘要

Summary Objective The purpose of the present study was to analyze the effects of ferrous sulfate in different trimesters of pregnancy on oxidative stress and p-NF-κB p65 in the placenta. Material and methods Twenty-four female Wistar rats were divided into four groups: the control group (no treatment), the group treated with ferrous sulfate from the first trimester (the first day of pregnancy), the second trimester (the eighth day of pregnancy) and the third trimester (the fifteenth day of pregnancy). Malondialdehyde levels as a marker of oxidative stress in the placenta was analyzed by a spectrophotometer. Placental expression of p-NF-κB p65 was analyzed by western blotting. Results Placental MDA levels were significantly higher for the three groups of ferrous sulfate administration relative to the group without ferrous sulfate administration (p?