Investigation of a new signalling pathway linking deltaD to ciliogenesis - the role of rabconnectin3

摘要

Recently, using the zebrafish mutant for the deltaD gene(after eight or aei-/-), our group has showed that Notchsignaling was involved in the control of cilia length in thecells of the fish laterality organ, the Kupffer’s Vesicle (KV)(Lopes el al. 2010). Further research based on microarrayscreening allowed the discovery of several genes with differentialexpression in KV cells from aei-/- mutants comparedto WT embryos. One of these genes, rabconnectin3or rc3 (ENSDARG00000091293) was found to be severelydownregulated. Homologs of this gene have recently beenassociated with Notch signaling in Drosophila and mammaliancells (Yan et al. 2009; Sethi et al. 2010, respectively)through the regulation of the V-ATPase activity. Furthermore,the activity of this pump has also been associatedwith the ciliogenesis in the KV (Chen et al. 2011). Using aMorpholino against rc3, we caused a decrease in the cilialength of the KV. We propose that the decrease in thecilia size present in the KV of the aei-/- mutants is causedby the deregulation of the transcription of genes such asrc3 and not necessarily by the disruption of the Notchsignaling.