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首页> 外文期刊>Canadian Journal of Veterinary Research >Comparison of 2 enzyme immunoassays and a radioimmunoassay for measurement of progesterone concentrations in bovine plasma, skim milk, and whole milk
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Comparison of 2 enzyme immunoassays and a radioimmunoassay for measurement of progesterone concentrations in bovine plasma, skim milk, and whole milk

机译:比较两种酶免疫法和放射免疫法测定牛血浆,脱脂牛奶和全脂牛奶中孕酮的浓度

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The objective of this study was to compare 2 enzyme immunoassays (EIAs) with a radioimmunoassay (RIA) as to sensitivity and accuracy in the measurement of the progesterone (P4) concentration in bovine plasma, skim milk, and whole milk. The 72 samples from 24 lactating dairy cows expected to have either a high P4 concentration (cows in diestrus or pregnant) or a low P4 concentration (cows in estrus or anestrus) were analyzed by RIA, solid-phase EIA (SPEIA), which included a solvent extraction step, or direct EIA (DEIA) without solvent extraction. The overall mean concentrations of P4 did not differ (P < 0.4) among the assays. However, for the cows that were in diestrus or pregnant, the mean P4 concentrations (and standard error) were higher (P < 0.03), regardless of sample type, with RIA than with SPEIA, at 7.3 (0.7) and 6.1 (0.6) ng/mL, respectively. When only the high-P4 samples analyzed by RIA were compared, the mean P4 concentration was higher (P < 0.001) in whole milk than in skim milk, at 9.8 (1.0) and 4.1 (0.7) ng/mL, respectively. Although the mean P4 concentrations in the low-P4 samples did not differ (P < 0.80) among assays, the proportions of cows with a P4 concentration ≥ 1 ng/mL were 3%, 14%, and 44% for RIA, SPEIA, and DEIA, respectively (P < 0.01; DEIA > SPEIA > RIA).
机译:这项研究的目的是比较两种酶免疫测定法(EIA)和放射免疫测定法(RIA)在测定牛血浆,脱脂乳和全脂乳中孕酮(P4)浓度方面的敏感性和准确性。通过RIA固相EIA(SPEIA)分析了来自24头泌乳奶牛的72个样本,这些样本的P4浓度高(雌性或孕期的母牛)或P4浓度低(发情或发情的母牛)。溶剂萃取步骤,或不进行溶剂萃取而直接进行EIA(DEIA)。在所有测定中,P4的总体平均浓度没有差异(P <0.4)。但是,对于处于绝育期或妊娠期的母牛,无论样品类型如何,RIA的平均P4浓度(和标准误)均高于SPEIA,分别为7.3(0.7)和6.1(0.6)(P <0.03) ng / mL。仅比较通过RIA分析的高P4样品时,全脂牛奶中的P4平均浓度比脱脂牛奶中的平均P4浓度高(P <0.001),分别为9.8(1.0)和4.1(0.7)ng / mL。尽管各试验中低P4样品中的P4平均浓度没有差异(P <0.80),但RIA,SPEIA,P4浓度≥1 ng / mL的母牛比例分别为3%,14%和44%,和DEIA(P <0.01; DEIA> SPEIA> RIA)。

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