Exploration on Mixed Cultivation of iLactobacillus, Yeast and Bacillus subtilis/i

摘要

To study on mixed fermentation of probiotics, bacterial cultivation was carried out firstly. It was showed that the optimal pH values for growth of iLactobacillus, Yeast/i and iBacillus subtilis/i were 6.5, 6.0 and 7.0, respectively. And the suitable temperature for all of them was about 31°C. Then under selective conditions pH 6.0, 31°C and 160 r/min, three probiotics were cultivated by taking iLactobacillus/i as main bacteria and the others as auxiliaries. It was found that the desired bacterial number ratio of iLactobacillus: Yeast: Bacillus/i for mixed cultivation was 1:2:2 and the OD value of mixed bacterial solution achieved at 2.27 after three days.