The -31 G/C promoter gene polymorphism of surviving in Turkish colorectal cancers patients

摘要

IntroductionSurvivin (also known as birc5) is the first protein discovered among the apoptosis-regulating gene family referred to as inhibitor of apoptosis proteins (IAPs). It is expressed and controlled during cellular differentiation and development in human beings. Survivin expression has been shown in a number of cancers and has been associated with cancer development.Objective In our study, we compared with blood samples as our control and normal- tumoural tissue samples, which obtained from the same tissue of 100 cases diagnosed with colorectal cancer, at Department of Pathology, Istanbul University.MethodsThe present study employed PCR-RFLP to identify the -31 G/C polymorphism in the promoter region of the survivin gene.? Distribution of the survivin polymorphism was compared between control and tumoural tissue samples using the chi-square test.Results Comparison of all samples revealed that there was significant difference in distribution of survivin promoter -31G/C between control group and tumour and normal tissue of the patient group (p0.05). When genotypes of the control and tumour tissues were compared according to gender, there was no statistically significant difference in the distribution of survivin promoter -31G/C in females p=0.420 or males p=0.309. ConclusionA significant difference was seen in distribution of C allele in tumour tissue compared to normal tissue.