Alterations in Microfilamentous and Microtubular Structures Accompanied by Cell Growth of SV40-Transformed Clonal Macrophages, Line 28-12 and its Subline

摘要

References(32) Cited-By(4) Simian virus 40 (SV40)-transformed macrophages, clonal line 28-12, and its subline, 28-12(Ara), were used to study morphological changes associated with their growth. Actively growing 28-12 cells were SV40 T-antigen-positive and had thick bundles of microfilaments, a few micro-tubules, and immature nuclei, whereas stationary 28-12 cells had no SV40 T-antigen or a markedly reduced amount of it, and possessed a network pattern of microfilaments similar to that of cultivated macrophages, many microtubules and mature nuclei. 28-12(Ara) cells had a low growth rate, a slightly reduced amount of SV40 T-antigen, a network pattern of microfilaments, significantly fewer microtubules and moderately mature nuclei.These results suggest that the high growth rate, which is possibly induced by viral products, converts the microfilament distribution from a network to a bundle pattern, the microtubule assembly in the pseudopodia from large to small quantities and the nuclear profile from a mature to an immature one.