Orai/CRACM1 and K Ca 3.1 ion channels interact in the human lung mast cell plasma membrane

摘要

Background Orai/CRACM1 ion channels provide the major Ca 2+ influx pathway for FcεRI-dependent human lung mast cell (HLMC) mediator release. The Ca 2+ -activated K + channel K _Ca 3.1 modulates Ca 2+ influx and the secretory response through hyperpolarisation of the plasma membrane. We hypothesised that there is a close functional and spatiotemporal interaction between these Ca 2+ - and K + -selective channels. Results Activation of FcεRI-dependent HLMC K _Ca 3.1 currents was dependent on the presence of extracellular Ca 2+ , and attenuated in the presence of the selective Orai blocker GSK-7975A. Currents elicited by the K _Ca 3.1 opener 1-EBIO were also attenuated by GSK-7975A. The Orai1 E106Q dominant-negative mutant ablated 1-EBIO and FcεRI-dependent K _Ca 3.1 currents in HLMCs. Orai1 but not Orai2 was shown to co-immunoprecipitate with K _Ca 3.1 when overexpressed in HEK293 cells, and Orai1 and K _Ca 3.1 were seen to co-localise in the HEK293 plasma membrane using confocal microscopy. Conclusion K _Ca 3.1 activation in HLMCs is highly dependent on Ca 2+ influx through Orai1 channels, mediated via a close spatiotemporal interaction between the two channels.