Value of Crossmatch Tests for Verifying the Compatibility in Dog Blood Transfusion Therapy

摘要

In case of pets, dogs and cats transfusions are already a current practice, beeing an intensive therapy procedure. In a group of dogs that underwent intensive therapy hole blood transfusion was used based on major and minor Crossmatch test results in order to establishing blood compatibility. The slide and tube techniques used were original and taken from different authors. Using 15 potential donors, 5 patients were transfused, one of them diagnosed with osteosarcoma. In 4 of the patients, all donor tested were compatible and they recovered after blood transfusion. Incompatibility problems appeared in the patient with osteosarcoma, for which 11 potential donors were tested and among them only 2 were compatible, the others showed positive agglutination reaction in major crossmatch In patients with positive reactions of incompatibility, the results were relevant in both variants (slide, tube), meaning the high level of plasmatic alloantibodies. After the evaluation of blood compatibility using major and minor crossmatch tests it was decided to opt for blood transfusion which made possible the recovery of 4 out of 5 dogs undergoing intensive therapy. The development was fatal for the patient with osteosarcoma because of the severe complications caused by the cancer. The highly positive major crossmatch results obtained in the patient with osteosarcoma and 9 potential donors, who never received a transfusion, suggest the possibility of producing alloantibodies to some foreign cancer induced proteins, cross-reactant with common erythrocyte antigens.