The production of tannase by Aspergillus tamarii was studied in submerged cultures. The fungus produced an extracellular tannase after two days of growth in mineral medium containing tannic acid, gallic acid and methyl gallate as carbon source. The best result was obtained using gallic acid as inducer (20.6 U/ml). The production of enzyme was strongly repressed by the presence of glucose. Crude enzyme was optimally active at pH 5.0 and 30o C. The enzyme was stable in a large range of pH and up to the temperature of 45o C.
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机译:在水下培养中研究了塔马里曲霉生产鞣酸酶的过程。在含有单宁酸,没食子酸和没食子酸甲酯作为碳源的矿物质培养基中生长两天后,真菌产生了胞外鞣酸酶。使用没食子酸作为诱导剂(20.6 U / ml)可获得最佳结果。葡萄糖的存在强烈抑制了酶的产生。粗酶在pH 5.0和30o C时具有最佳活性。该酶在很大的pH范围内和最高45 o C的温度下均稳定。
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