The production of tannase by Aspergillus tamarii was studied in submerged cultures. The fungus produced an extracellular tannase after two days of growth in mineral medium containing tannic acid, gallic acid and methyl gallate as carbon source. The best result was obtained using gallic acid as inducer (20.6 U/ml). The production of enzyme was strongly repressed by the presence of glucose. Crude enzyme was optimally active at pH 5.0 and 30 degrees C. The enzyme was stable in a large range of pH and up to the temperature of 45 degrees C.
展开▼
机译:在深水培养中研究了塔马里曲霉产生鞣酸酶的过程。在含有单宁酸,没食子酸和没食子酸甲酯作为碳源的矿物培养基中生长两天后,该真菌产生了一种细胞外鞣酸酶。使用没食子酸作为诱导剂(20.6 U / ml)可获得最佳结果。葡萄糖的存在强烈抑制了酶的产生。粗酶在pH 5.0和30摄氏度下具有最佳活性。该酶在很大的pH范围和最高45摄氏度的温度下均稳定。
展开▼