The Antimicrobial Activity of Ethanol Extract of Thymus vulgaris on Salmonella typhi in Rabbets

摘要

The aim of this study was in vivo antimicrobial activity of ethanol extract of Thymus vukgaris on Salmonella typhi isolated from patients in Sulaymani city, Iraq. Thirty Salmonella entereca serova typhi were isolated from blood of patients suffering from typhoied fever in indor and outdor of Sulaymani city-Iraq. The isolates were identified through cultural, morphological and biochemical examination, in addition to API20E system. Susceptibility test to eleven antimicrobials were performed for all isolates. The isolates were grouped to 7 antibiogram; The S. enterica isolates were 100% resist to Amp, Cef, Chl, Gm, Tet and Tri antibiotics, or resist to at least one more antibiotics. The Minimum Inhibition Concentration (MIC) of ethanol extract for Thymus vulgaris was determined (2500 μg/mL). Treating local rabbits (WBC count of 3000-4000) with MIC of T. vulgaris extract, after orally infected with more resistant isolate S. typhi S5, and appearance of disease septum with decreasing of WBC count and lymphocyte, while neutrophil increased for all groups. After treating the infected rabbits with combination of plant extract and Gentamycin after two month from infection, the numbers of survived rabbits were more than other treatments.