Antibodies to hepatitis B virus surface antigen and interleukin 12 and interleukin 18 gene polymorphisms in hemodialysis patients

摘要

Background The interleukin (IL)18 rs360719 CC genotype is associated with the development of antibodies to hepatitis B virus surface antigen (anti-HBs) in hemodialysis (HD) patients. IL18 shares biological properties with IL12 in promoting the T-hepler 1 (Th1) system. We studied whether polymorphisms in the IL12A 3` untranslated region (UTR) and IL12B 3`UTR may contribute to anti-HBs development (titre ≥ 10?IU/L) in HD patients either individually or jointly with the IL18 polymorphism. Methods In 518 HD patients and 240 controls the IL12A rs568408 3’UTR G?>?A polymorphism was genotyped by high-resolution melting curve analysis. Polymerase chain reaction restriction fragment length polymorphism was used to detect the IL12B rs3212227 3’UTR A?>?C and IL18 -1297?T?>?C rs360719 polymorphisms. The associations between the IL12A, IL12B and IL18 genotypes and the risk of impaired anti-HBs development were estimated by computing the odds ratios and their 95% confidence intervals using logistic regression analysis. Results In the logistic regression analysis, the higher frequency of rs360719 CC individually (2.9% in 207 patients without anti-HBs development vs 8.0% in 311 patients with anti-HBs development, p?=?0.009) and of rs360719 CC combined with rs568408 GG (p?=?0.048), rs568408 GA (p?=?0.035), rs568408 GG/AA (p?=?0.034) or rs3212227 AA (p?=?0.046) was associated with an increased chance for the development of anti-HBs in HD patients. Patients bearing both rs568408 AA and rs360719 TT had a 10.9-fold or 8.9-fold lower chance, respectively, to develop anti-HBs compared with those carrying any other genotype (p?=?0.005) or those who had both wild-type rs568408 GG and rs360719 TT (p?=?0.011). Carriers of both rs3212227 CC and rs360719 TC had a 4.6-fold lower chance for anti-HBs development than carriers of any other genotype (p?=?0.042). Conclusion Development of anti-HBs in HD patients is associated with gene polymorphisms of interleukins involved in the Th1 system.