A histological study of the effect of exogenous melatonin on gentamicin induced structural alterations of proximal tubules in rats

摘要

Th e aim of this research was to assess the reactive changes of rat proximal tubules caused by gentamicin and the eff ect of relatively low doses of melatonin. ?? adult male Wistar rats were distributed into six groups of equal size which all received one of the following daily intraperitoneal injections: vehicle (?? ethanol in Ringer solution) during ?? days (C); gentamicin (?? mg/kg) during ? days (G), two groups which concomitantly received gentamicin (?? mg/kg) during ? days and melatonin in two diff erent test doses (? or ?? mg/kg) during ?? days (GM?, GM?) and two groups treated only with melatonin in two diff erent doses (? or ?? mg/kg) during ?? days (M?, M?). Histological analysis included qualitative and semi-quantitative light microscopy analysis of proximal tubules. Exogenous melatonin had no signifi cant eff ect on the microstructure, independently of dosis. Th e changes of proximal tubules microstructure induced by gentamicin were expressed in the form of granulovacuolar degeneration, necrosis and desquamation. Th e grade of proximal tubular changes was smaller in animals who besides gentamicin received melatonin. Melatonin has a dose dependent protective eff ect on the structural alterations of proximal tubules of the kidney induced by gentamicin.