B-type natriuretic peptide and adiponectin releases in rat model of myocardial damage induced by isoproterenol administration

摘要

B-type natriuretic peptide (BNP) and adiponectin play important role in the cardiovascular homeostasis regulation. We investigated BNP and adiponectin serum levels followed by isoproterenol (ISO) administration to rats and explored the relationship between them. Cardiac troponin I (cTnI) blood level was used as biochemical evidence of myocardial damage development. Adult male Wistar rats (average body weight ???.??± ??.?? g) were distributed into groups: control group received saline (n=?) and ISO groups (n=??) treated with ISO (subcutaneous single dose ??? mg/kg of rat body weight). ISO group was divided into two groups according to the time of BNP, adiponectin and cTnI determination: ISO I (n=?; ? hours after ISO administration); ISO II (n=?; ? hours after ISO administration). Blood for determination of parameters was taken from rat abdominal aorta. BNP, adiponectin and cTnI were determined by ELISA method. Data were statistically analysed by using SPSS version?? computer program. P value less ?.?? was considered statistically signifi cant. Blood BNP and adiponectin were lower at ? hours after ISO administration in comparison with control group (p=?.??? for BNP and p=?.??? for adiponectin). Four hours after ISO administration, we have noted signifi cant elevation of both parameters compared to ISO I group (p=?.??? for BNP; p=?.?? for adiponectin). Test of correlation have showed signifi cant relation between their blood levels during experimental period (rho=?.???; p=?.??). BNP and adiponectin are not simple indicators of myocardial damage development. Th ey have possible associated and additive eff ects in cardiovascular homeostasis regulation.