Uni-axial stretch induces actin stress fiber reorganization and activates c-Jun NH 2 terminal kinase via RhoA and Rho kinase in human bladder smooth muscle cells

摘要

Background Excessive mechanical overload may be involved in bladder wall remodelling. Since the activity of Rho kinase is known to be upregulated in the obstructed bladder, we investigate the roles of the RhoA/Rho kinase pathway in mechanical overloaded bladder smooth muscle cells. Methods Human bladder smooth muscle cells were stimulated on silicon culture plates by 15?% elongated uni-axial cyclic stretch at 1 Hz. The activity of c-Jun NH₂-terminal kinase was measured by western blotting and actin stress fibers were observed by stained with phallotoxin conjugated with Alexa-Fluor 594. Results The activity of c-Jun NH₂-terminal kinase 1 peaked at 30?min (4.7-fold increase vs. before stretch) and this activity was partially abrogated by the RhoA inhibitor, C3 exoenzoyme or by the Rho kinase inhibitor, Y-27632. Stretch induced the strong formation of actin stress fibers and these fibers re-orientated in a direction that was perpendicular to the stretch direction. The average angle of the fibers from the perpendicular to the direction of stretch was significantly different between before, and 4?h after, stretch. Actin stress fibers reorganization was also suppressed by the C3 exoenzyme or Y-27632. Conclusions Bladder smooth muscle cells appear to have elaborate mechanisms for sensing mechanical stress and for adapting to mechanical stress overload by cytoskeletal remodeling and by activating cell growth signals such as c-Jun NH₂-terminal kinase via RhoA/Rho kinase pathways.