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Identification of molecular markers linked to Fusarium ear rot genes in maize plants Zea mays L

机译:玉米中玉米镰刀菌腐烂基因相关分子标记的鉴定

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ABSTRACT Two maize populations (GE440 ???? Sd7 and NC300 ???? Gm1002) were used in this study. Molecular markers like simple sequence repeat (SSR) and sequence-tagged site (STS) were used to identify resistance genes linked to Fusarium ear rot ( Fusarium verticillioides ). Also, quantitative trait locus (QTL) mapping was used to detect resistance genes on maize chromosomes. The co-dominant microsatellite markers (bnlg1063, umc2082, bnlg1621, umc2013, bnlg1740, umc2059 and SSR85) and STS (STS06) were amplified polymorphic bands and candidate markers linked to Fusarium ear rot resistance genes in maize. Seven QTLs were identified ranging from 2 to 7 for each trait. QTLs were distributed on two chromosomes and associated with grain yield/main ear and 100 kernel weight. The present study indicated that microsatellite and STS primers might be useful for developing improved cultivars.
机译:摘要本研究使用了两个玉米种群(GE440→Sd7和NC300→Gm1002)。简单序列重复序列(SSR)和序列标记位点(STS)等分子标记用于鉴定与镰刀菌腐烂(Fusarium v​​erticillioides)相关的抗性基因。同样,使用定量性状基因座(QTL)作图来检测玉米染色体上的抗性基因。将共显性微卫星标记(bnlg1063,umc2082,bnlg1621,umc2013,bnlg1740,umc2059和SSR85)和STS(STS06)扩增为多态性谱带,并选择了与玉米镰刀菌耐腐基因相关的候选标记。每个性状鉴定出七个QTL,范围从2至7。 QTL分布在两个染色体上,并与谷物产量/主穗和100粒重相关。本研究表明,微卫星和STS引物可能对开发改良的品种有用。

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