Molecular Genetic Map Construction and QTL Analysis for Fruit Maturation Period in Litchi

摘要

A hybrid F1 population of ?￠????Maguili x Jiaohesanyuehong?￠???? was created by crossing between the very late ripening cultivar ?￠????Maguili?￠???? as the maternal parent and the very early ripening cultivar ?￠????Jiaohesanyuehong?￠???? as the paternal parent. Seventy-six randomly chosen F1 individuals were used as the mapping population. SRAP and AFLP molecular marker analysis were carried out with the mapping population and the two parents. Molecular genetic maps for both parents were built. The ?￠????Maguili?￠???? map contained 179 loci which fell into 16 linkage groups. The coverage of the map was 610.55cM with an average distance of 3.75cM between markers. The ?￠????Jiaohesanyuehong?￠???? map consisted of 82 loci allocated into 15 linkage groups. The map covered 548.09cM and had a mean distance of 8.18cM between markers. IM method was used to determine QTLs for the fruit maturation period. Five QTLs for fruit maturation period were detected. Two QTLs were on LG ?￠????JHSYH2?￠????and ?￠????JHSYH4?￠???? of the paternal map and explained a contribution rate of 34.4% and 70.1% respectively. Three QTLs were on LG ?￠????MGL2?￠???? and ?￠????MGL5?￠????, and the contribution rate they can explain was between 38.3% and 65.8%.