Genetic diversity assessment using simple sequence repeats (SSR) and sequence-related amplified polymorphism (SRAP) markers in ramie

摘要

Simple sequence repeats (SSRs) and sequence-related amplified polymorphism (SRAP) were employed to assess the genetic diversity and relationship among 108 ramie cultivars, which were collected from China, Japan, Indonesia and Myanmar. A total of 183 polymorphic bands were produced by using 21 SSR and 20 SRAP primer pairs. The genetic similarity matrix was obtained by using the similarity for qualitative data (SIMQUAL) subroutine of the Numerical Taxonomy and Multivariate Analysis System (NTSYS-pc) software statistical package, based on Jaccard's algorithms. The results showed that the similarity coefficient among the 108 ramie germplasms ranged from 0.22 to 0.89. By using principal components analysis (PCA) and unweighted pair-group method with arithmetic means (UPGMA), the 108 ramie germplasms were clustered into two categories. Shanon diversity index (SDI) was 1.1 and Nei's gene diversity index (NGDI) was 0.58 in the present study. When comparing their indexes, the genetic diversity in the different provinces was as follows: Guizhou (SDI 1.14, NGDI 0.61) > Jiangxi (SDI 1.11, NGDI 0.60) > Guangxi (SDI 1.05, NGDI 0.59) > Hunan (SDI 1.04, NGDI 0.58) > Hubei (SDI 1.02, NGDI 0.58) > Chongqing (SDI 0.96, NGDI 0.54). There was an abundant genetic diversity in ramie accessions growing in the China's Changsha field GenBank of ramie. Guizhou province had the highest genetic diversity and thus may be the origin centre of ramie's genetic diversity. The relationship between germplasms and geographic distribution was poor.