Statistical optimization of ATPi system and extraction of papain in desktop ENZextractor

摘要

ATPi- A concept of process integration which is meant for single step purification process inBioseparation industry is designed for the Industrial enzyme extraction. Integrating protein affinity groups i.e., ligands to one of the phase forming polymer in ATP system is termed as integrated aqueous two phase system (ATPi) in this work. The ATPi system is composed of PEG+ 1-butyl phosponiumbromide as ligand andAmmonium sulphate. RSMthrough CCD analysis was used to identify optimal values of extraction parameters for papain separation from carica papaya fruit latex. The optimized parameters fromlaboratory scale was applied in a 3L pilot scale, Laboratory designed ENZextractor with a working volume of 2L. It was proved that 96% accuracy of model and 92% recovery was achieved through the ENZextractor.