Reconfiguring the quorum sensing regulator SmcR of Vibrio vulnificus to control virulence via endophytic fungal compound-Pyrrolo [1, 2-a] pyrazine-1, 4-dione, hexahydro-3-(2-methylpropyl)

摘要

Vibrio vulnificus is a mortiferous pathogen accounting for 95% of all deaths related to sea foods. The objective of this study is to isolate secondary metabolites from endophytic fungi, that binds to SmcR (a quorum sensing regulator) in such a way that it down regulates metallo protease gene with no alterations made in the domain of SmcR that represses hemolysin. The extract was analyzed by GC-MS, from which seven secondary metabolites were identi?ed by comparing the mass spectral data withNIST library. Potential lead (VVSI3) was found through docking studies that could effectively bind to SmcR and curb its action. Based on this, in vitro work was carried out which exhibited a noteworthy drop-off in protease activity of Vibrio vulnificus, proving it to be a potential drug lead.