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Bioethanol production from sugarcane leaf waste: Effect of various optimized pretreatments and fermentation conditions on process kinetics

机译:甘蔗叶废料生产生物乙醇:各种优化的预处理和发酵条件对过程动力学的影响

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This study examines the kinetics of S. cerevisiae BY4743 growth and bioethanol production from sugarcane leaf waste (SLW), utilizing two different optimized pretreatment regimes; under two fermentation modes: steam salt-alkali filtered enzymatic hydrolysate (SSA-F), steam salt-alkali unfiltered (SSA-U), microwave salt-alkali filtered (MSA-F) and microwave salt-alkali unfiltered (MSA-U). The kinetic coefficients were determined by fitting the Monod, modified Gompertz and logistic models to the experimental data with high coefficients of determination Rsup2/sup 0.97. A maximum specific growth rate (μ submax/sub ) of 0.153?hsup?1/sup was obtained under SSA-F and SSA-U whereas, 0.150?hsup?1/sup was observed with MSA-F and MSA-U. SSA-U gave a potential maximum bioethanol concentration (Psubm/sub) of 31.06?g/L compared to 30.49, 23.26 and 21.79?g/L for SSA-F, MSA-F and MSA-U respectively. An insignificant difference was observed in the μ submax/sub and P subm/sub for the filtered and unfiltered enzymatic hydrolysate for both SSA and MSA pretreatments, thus potentially reducing a unit operation. These findings provide significant insights for process scale up.
机译:这项研究利用两种不同的优化预处理方案研究了酿酒酵母BY4743生长和甘蔗叶废料(SLW)生产生物乙醇的动力学。在两种发酵模式下:蒸汽盐-碱过滤的酶水解物(SSA-F),蒸汽盐-碱未过滤(SSA-U),微波盐-碱过滤(MSA-F)和微波盐-碱未过滤(MSA-U) 。通过将Monod模型,改进的Gompertz模型和logistic模型拟合到具有高测定系数R 2 max )为0.153?h ?1 ,而0.150?h ?1 <使用MSA-F和MSA-U观察到/ sup>。 SSA-U的潜在最大生物乙醇浓度(P m )为31.06?g / L,而SSA-F,MSA-F和MSA-U分别为30.49、23.26和21.79?g / L。 。对于SSA和MSA预处理,已过滤和未过滤的酶水解液的μ max 和P m 差异均不显着,因此有可能减少单元操作。这些发现为过程扩展提供了重要的见识。

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