The fabrication of magnetic particle-based chemiluminescence immunoassay for human epididymis protein-4 detection in ovarian cancer

摘要

The magnetic particles have a significant influence on the immunoassay detection and cancer therapy. Herein, the chemiluminescence immunoassay combined with the magnetic particles (MPCLIA) was presented for the clinical determination and analysis of human epididymis protein 4 (HE4) in the human serum. Under the optimized experiment conditions, the secure MPCLIA method can detect HE4 in the broader range of 0–1000pmol/L, with a lower detection limit of 1.35pmol/L. The satisfactory recovery rate of the method in the serum ranged from 83.62% to 105.10%, which was well within the requirement of clinical analysis. Moreover, the results showed the good correlation with enzyme-linked immunosorbent assay (ELISA), with the correlation coefficient of 0.9589. This proposed method has been successfully applied to the clinical determination of HE4 in the human serum. Highlights ? A high-sensitivity, relatively simple and rapid MPCLIA has been successfully developed for the clinical determination of HE4 in human serum. ? The quantitative determination for HE4 in the serum samples was with satisfactory recoveries ranging from 83% to 105%. ? The magnetic particle-based chemiluminescence immunoassays could be used for the quantitative detection of other tumor markers. ? This assay provides apparent advantages and shows great potential in the clinical diagnosis.