Influence of sexual season on implantation of microinjected embryos and birth rate of new born-transplant in experiments on creating transgenic goats carrying human lactoferrin gene

摘要

The article presents the results of the study on the viability of microinjected embryos in goats, as well as determiningthe level of engraftment of transplanted embryos, depending on the season. Also, a new scheme of getting even-agedoocytes at the stage of two pronuclei was proposed. Polyovulation in donor goats amounted to 16.4 of corpus luteumper head. In addition, 11.8 oocytes (embryos)/head were recovered, 87.5% of them being presented by the fertilizedoocytes at the stage of two pronuclei. During the transplantation of embryos after microinjection of gene construction,their acceptability was low and amounted to an average of 16.3% (lim 8.3-27%). In sexual season, the acceptabilitywas 3.25 times higher than in nonsexual season. Fetal lysis of already implanted embryos was averaged to 13.7%.