Effects of zirconium oxide nanoparticles on bacteria isolated from activate sludge detected by RAPD analysis

摘要

This research has been conducted to determine the genotoxicity of nano-ZrO2 towards Pseudomonas putida and Aeromonas hydrophila bacteria using randomly amplified polymorphic DNA (RAPD)-PCR method. Results obtained for the nanocompound were compared with those for ZrO2 macro form. The nanocompound caused changes in the genetic material of bacteria. P. putida was more sensitive to nano-ZrO2 than A. hydrophila. Degree of genetic similarity (S) of obtained profiles bands for primer OPA2 differed from the results obtained for the negative control by more than 30%, while from positive control - only by 12%. In turn, the largest decrease in genetic stability (GTS) was 75.1%. The results also showed that the nano-ZrO2 can induce modifications of the genetic material to a greater extent than the same compounds in their macro form. The obtained data confirmed that RAPD-PCR can be successfully used to monitor potentially genotoxic effects of various nanoparticles.