Ten novel polymorphic microsatellite loci of Red-spotted grouper (Epinephelus akaara) revealed from full-sib progeny and unrelated individuals

摘要

We isolated 12 candidate microsatellite loci from a small insert genomic DNA library of the Red-spotted grouper, Epinephelus akaara. Evaluation of full-sib progeny and unrelated individuals revealed that two of the 12 loci had distorted segregation states based on lack of adherence to Mendelian laws and Hardy–Weinberg equilibrium. We assessed polymorphism in the 10 loci using 20 unrelated individuals from a single population. The number of alleles per locus ranged from 2 to 19, allelic richness ranged from 2 to 17.6, and the observed heterozygosity ranged from 0.35 to 1.00 with no evidence of linkage disequilibrium.