Ten novel polymorphic microsatellite loci of Red-spotted grouper (Epinephelus akaara) revealed from full-sib progeny and unrelated individuals

摘要

We isolated 12 candidate microsatellite loci from a small insert genomic DNA library of the Red-spotted grouper, Epinephelus akaara. Evaluation of full-sib progeny and unrelated individuals revealed that two of the 12 loci had distorted segregation states based on lack of adherence to Mendelian laws and Hardy-Weinberg equilibrium. We assessed polymorphism in the 10 loci using 20 unrelated individuals from a single population. The number of alleles per locus ranged from 2 to 19, allelic richness ranged from 2 to 17.6, and the observed heterozygosity ranged from 0.35 to 1.00 with no evidence of linkage disequilibrium.