Separation and Purification of Annexin B1, a Novel Ca2+-Dependent Phospholipid Binding Protein, Expressed in Escherichia coli by Ion-Exchange Chromatography

Yi Zhang; Yingjun Guo; Yuanjian Gao; Shu Zhang; Hongli Yan; Shuhan Sun

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Separation and Purification of Annexin B1, a Novel Ca2+-Dependent Phospholipid Binding Protein, Expressed in Escherichia coli by Ion-Exchange Chromatography

机译：离子交换色谱法分离和纯化膜联蛋白B1，一种新型的Ca2 +依赖性磷脂结合蛋白，在大肠杆菌中表达

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Annexin B1 has been cloned in Escherichia coli (E. coli) K802 in previous works. After temperature shift induction the protein was expressed as soluble form. Then a separation method for annexin B1 from E. coli cells by ion-exchange chromatography has been developed and validated. The method exhibited a good reproducibility and is easy to operate. It has shown that annexin B1 isolated by chromatography had high anticoagulant activity.

机译：在以前的工作中，膜联蛋白B1已被克隆到大肠杆菌（E. coli）K802中。温度转变诱导后，蛋白质以可溶形式表达。然后开发并验证了通过离子交换色谱从大肠杆菌细胞中分离膜联蛋白B1的方法。该方法具有良好的重现性并且易于操作。已经表明，通过色谱法分离的膜联蛋白B1具有高的抗凝活性。

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来源
《Chromatographia》 |2003年第12期|713-716|共4页
作者
Yi Zhang; Yingjun Guo; Yuanjian Gao; Shu Zhang; Hongli Yan; Shuhan Sun;
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作者单位

Department of Medical Genetics The Second Military Medical University;

Department of Medical Genetics The Second Military Medical University;

Department of Medical Genetics The Second Military Medical University;

Department of Medical Genetics The Second Military Medical University;

Department of Medical Genetics The Second Military Medical University;

Department of Medical Genetics The Second Military Medical University;

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正文语种 eng
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关键词
Column liquid chromatography Ion-exchange chromatography Annexin B1 Escherichia coli;

机译：柱液相色谱离子交换色谱Annexin B1大肠杆菌;

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1. Non-fusion expression in Escherichia coli, purification, and characterization of a novel Ca2+- and phospholipid-binding protein annexin B1 [J] . Zhang Y., Guo YJ., Sun SH., Protein Expression and Purification . 2004,第1期

机译：大肠杆菌中的非融合表达，新型Ca2 +和磷脂结合蛋白Annexin B1的纯化和表征
2. Web-based applet is a learning tool that simulates ion-exchange chromatography purification of overexpressed proteins from Escherichia coli cell lysate [J] . Usher K.C., Barrette-Ng I.H. Journal of Chemical Education . 2012,第4期

机译：基于Web的applet是一种学习工具，可以模拟离子交换色谱法纯化大肠杆菌细胞裂解液中过表达的蛋白质
3. Expression and purification of pneumococcal surface protein a of Glade 4 in Escherichia coli using hydroxylapatite and ion-exchange column chromatography [J] . Xi Hualong, Yu Jinfei, Sun Qing, Protein Expression and Purification . 2018,第期

机译：使用羟基磷灰石和离子交换柱色谱法在大肠杆菌中肺球表面蛋白A的表达和纯化
4. Chromatography purification of the promising biomaterial: elastin-like protein genetically expressed in Escherichia coli [C] . Q Wei, X X Wang, S S Yang International Conference on New Material and Chemical Industry . 2019

机译：色谱法纯化了有前途的生物材料：ELASTIN样蛋白在大肠杆菌中遗传表达
5. The purification of proteins having properties of an N-methyl-D-aspartate (NMDA) receptor from brain synaptic membranes and characterization of a glycine-binding protein expressed in Escherichia coli. [D] . Babcock, Kent Kincaid. 1994

机译：从脑突触膜纯化具有N-甲基-D-天冬氨酸（NMDA）受体特性的蛋白质，并表征在大肠杆菌中表达的甘氨酸结合蛋白。
6. Expression purification and characterization of a novel Ca2+- and phospholipid-binding protein annexin B2 [O] . Yi-Ming Lu, Na Wang, Jun-Jie Wang, -1

机译：新型Ca2 +和磷脂结合蛋白Annexin B2的表达纯化和表征
7. Cytoplasmic high-level expression of a soluble, enzymatically active form of the Escherichia coli penicillin-binding protein 5 and purification by dye chromatography [O] . Mark P. G. LINDEN, Harald MOTTL, Wolfgang KECK 1992

机译：溶解的细胞质高液体表达，酶活性形式的大肠杆菌青霉素结合蛋白5和染料色谱法纯化

Separation and Purification of Annexin B1, a Novel Ca2+-Dependent Phospholipid Binding Protein, Expressed in Escherichia coli by Ion-Exchange Chromatography

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