Stationary-Phase Optimized Selectivity LC (SOS-LC): Separation Examples and Practical Aspects

摘要

Recently the principle of a novel approach was demonstrated:the stationary-phase optimized selectivity liquid chromatography (SOS-LC). In this paper some practical aspects are given and applications are presented to demonstrate the effect and possibilities of this novel procedure. Applying the same mobile phase and constant operating conditions the retention factors (k) of the model compounds have to be determined on three different single stationary phases. The optimal stationary phase combination can be predicted using serially connected columns and the principle of the ''PRISMA'' model, applied so far for mobile phase optimization. Generally the overall selectivity of this separation is better than that of any individual stationary phase. By use of different combinations the selectivity can be finely adjusted and even different elution order might be achieved. As new results of the application of SOS-LC complete separations of 7 flavonoids and 12 pesticides are demonstrated. The conclusions of the experiments dealing with the effect of the physical order of the different stationary phase segments within the serially connected column is also discussed. Finally, the SOS-LC optimization procedure is demonstrated in a form of a flow-chart.