Simultaneous Determination of Fluoroquinolones and Sulfonamides in Chicken Muscle by LC with Fluorescence and UV Detection

摘要

A simple, rapid and sensitive liquid chromatographic method with programmable fluorescence and ultraviolet detection was developed and validated for simultaneous determination of seven fluoroquinolones (marbofloxacin, ofloxacin, ciprofloxacin, lomefloxacin, danofloxacin, enrofloxacin and difloxacin) and four sulfonamides (sulfadiazine, sulfapyridine, sulfathiazole and sulfadimidine) in chicken muscle in a single run. The tissue sample was extracted with phosphate buffer (pH 6.0) and cleaned-up with a solid phase extraction cartridge. The mean recoveries for each drug in chicken muscle ranged from 78.0 to 105.2% with a relative standard deviation below 9.3% at 0.2–400 ng g−1 fortification levels. The limit of quantification was 0.2–4.0 ng g−1 for fluoroquinolones and 15.0 ng g−1 for sulfonamides.