Effect of nonoclonal antibody to bull sperm sp18 on murine fertilization and embryos development

摘要

Western blot analysis revealed that one IgG_1 monoclonal antibody(mAb)to sp18 family membrane proteins(Mr. 14,16 and 18 ku)of bovine sperm reacted faintly with protein bands of 14, 18,22,30 and 60 ku(reducing)in samples of mouse sperm. The mAb also reacted to protein of egg lysozyme. Using a laser confocal microscope, indirect immunofluorescence(IF)showed that the sp18 antigens were present in the posterior head of murine sperm.