Effects of metal ions on recombinant calcineurin A subunit

摘要

Effects of metal ions on activities and solution conformations of calcineurin A subunit have been examined. The ability of several metal ions to activate calcineurin A has been tested with Ni~2+ > Mn~2+ > Mg~2+/Ca~2+. The corresponding CD spectra and intrinsic fluorescent emission spectra show that calcineurin A exists in different metal ion-dependent conformation states. Effects of the different concentritions of Ni~2+ on activities and solution conformations of calcineurin A have been tested too.