Immunohistochemical localization of cytochrome P450 enzymes 2C and 4A in the normal rat brain

摘要

Conclusions P450 2C-11 protein, rather than P450 4A-2, 3 and 8, may be a candidate of brain P450 enzymes in the normal male rats. Objective To localize cytochrome P450 enzymes 4A and 2C in central nervous cells of normal male rats. Methods Eight drug/alcohol untreated normal Male rats (150-200 g of body weight) were treated by The optimized perfusion technique, then brain tissues were postfixed, paraffin-embedded and cut into series sections, which were labeled by the improved strept-avidin-biotin complex DAB-nickel enhancer (SABC-DAB-Ni) immunohistochemistry and hema- toxylin & eosin (H & E) stain techniques.