Expression of splice variants of CD44 mRNA and its significance in human primary hepatocellular carcinoma

摘要

Conclusion CD44 may contribute to the metastatic potential of PHCC. Objective To investigate the relationship be- Tween the expression of splice variants of CD44 (CD44v) and the metastasis of primary hepatocellular carcinoma (PHCC) in humans. Methods The expression of metastasis-associat- ed CD44v mRNA in 54 speciments of human PHCC was detected by reverse transcription and polymerase chain reaction (RT-RCR) method. Results CD44 mRNA was expressed in 50 (92.59/100) of the 54 specimens of PHCC, with moder- ate or over expression on 42 (77.78/100). Among the 42 specimens with moderate or over expression, deficient tumor capsules were fond in 25 (59.52/100), tumor emboli in tion of tumor capsule in 15 (35-71/100), tumor emboli in the portal vein in 34 (80.95/100), and daughter tumor modules in 29 (69.05/100).