Detection of Fas/APO-1 in six human urogenital malignant cell lines with flow cytometry

摘要

Conclusions Lower expression of Fas/APO-1 in urogenital malignant cell lines than that in normal cells might be the reason for occurrence and progression of urogenital malignant tumors. Objective To investigate the expression of Fas/APO-1 in urogenital tumor cell lines. With direct immunofluorescence, the expression of Fas/APO-1 in six urogenital malignant cell lines and one primary in vitro cultured normal renal fibroblast was detected by flow cytometry. Expression of Fa/APO-1 was detected in all six urogenital tumor cell lines, but with limited positive cell percentage and relatively lower fluores- cence intensity, compared with expression of Fas/APO-1 in normal control of primary in vitro cultured Renal fibroblast.