Clonal expansion T cells identified in acute monoblastic leukemia by CDR3 size analysis of TCR Vβrepertoire using RT-PCR and genescan

摘要

Conclusions T cell clonality expansion was found in AML-M5 cases and were tendetious in the Vβ2 subfamily, suggesting a the specific immune response for leukemia cell(M5)associated antigen and may display antileukemia activity. Objective To investigate the distribution and clonality of T cell receptor(TCR)Vβrepertoire in patients With acute monoblastic leukemia(AML-M5). Methods Expression of the TCR Vβreportoire was analyzed using reverse transcription-polymerase chain Reaction(RT-PCR), which amplified the complementarity determining region 3 of 24 TCR Vβgenes in Peripheral blood from 9 cases with acute myclogenous leukemia subtype 5 or acute monoblastic leukemia (AML-M).