Detection of p(14; 18) Chromosomal translocation In paraffin-embedded hepatocellular Carcinoma tissue by in situ PCR

摘要

Objective: to understand the relationship between (14;18) chromosomal translocation and hepatocellular carcinoma. Methods: Semi-nested in situ pCR (SNISPCR) technique was used to detected bcl-2/JH fusion gene in 40 cases of hepatocellular carcinoma (HCC). Results: Bcl-2/JH fusion gene was detected in 10 of 40 HCC. There were no significant differences in bcl-2/JH fusion formation between histopathological grades and metastases (P>0.05).