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首页> 外文期刊>Chemical Research in Chinese Universities >Direct MALDI-MS Analysis of Proteins Isolated by Liquid-phase Isoelectric Focusing Electrophoresis
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Direct MALDI-MS Analysis of Proteins Isolated by Liquid-phase Isoelectric Focusing Electrophoresis

机译：液相等电聚焦电泳分离蛋白质的直接MALDI-MS分析

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摘要

A liquid-phase isoelectric focusing electrophoresis system(Rotofor)was used as the prefractionation tool for the sample preparation in the MALDI-MS analysis of a protein mixture.Each fraction collected was then directly subjected to MALDI-TOF-MS analysis.By this approach,we are able to resolve two types of hemoglobins,A and C,which cannot be successfully separated by means of the traditional SDS-PAGE method.
机译：液相等电聚焦电泳系统（Rotofor）被用作蛋白质混合物MALDI-MS分析中样品制备的预分离工具，然后将收集的每个馏分直接进行MALDI-TOF-MS分析。 ，我们能够解决两种类型的血红蛋白，A和C，它们是传统SDS-PAGE方法无法成功分离的。

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  • 来源
    《Chemical Research in Chinese Universities》 |2005年第4期|p.436-438|共3页
  • 作者

    LIU Ning; LU Lei; ZHANG Xuan; WANG Zhi; SUN Ya-dong;

  • 作者单位

    Spectrometry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,P.R.China;

    Institute of Life Science and Technology,Jilin University,Changchun 130021,P.R.China;

    Key Laboratory of Molecular Enzymology and Engineering o;

    Spectrometry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,P.R.China;

    Institute of Life Science and Technology,Jilin University,Changchun 130021,P.R.China;

    Key Laboratory of Molecular Enzymology and Engineering o;

    Spectrometry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,P.R.China;

    Institute of Life Science and Technology,Jilin University,Changchun 130021,P.R.China;

    Key Laboratory of Molecular Enzymology and Engineering o;

    Spectrometry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,P.R.China;

    Institute of Life Science and Technology,Jilin University,Changchun 130021,P.R.China;

    Key Laboratory of Molecular Enzymology and Engineering o;

    Spectrometry,Changchun Institute of Applied Chemistry,Chinese Academy of Sciences,Changchun 130022,P.R.China;

    Institute of Life Science and Technology,Jilin University,Changchun 130021,P.R.China;

    Key Laboratory of Molecular Enzymology and Engineering o;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 化学;
  • 关键词

    Liquid-phase isoelectric focusing electrophoresis; MALDI-MS analysis; Hemoglobin A and C; Cyto-chrome c;

    机译：液相等电聚焦电泳;MALDI-MS分析;血红蛋白A和B;细胞色素c;

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